dna sanger sequencing facility (Thermo Fisher)
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Thermo Fisher
dna sanger sequencing facility
Dna Sanger Sequencing Facility, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+sanger+sequencing+facility/Deoxyribonucleic+acid/10__1007_slash_s10658___026___03185___7-47-35-44
Average 99 stars, based on 1 article reviews
Dna Sanger Sequencing Facility, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+sanger+sequencing+facility/Deoxyribonucleic+acid/10__1007_slash_s10658___026___03185___7-47-35-44
Average 99 stars, based on 1 article reviews
dna sanger sequencing facility - by Bioz Stars,
2026-10
99/100 stars
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Polymerase Chain Reaction:Article Title: Fusarium species associated with Euwallacea xanthopus in South Africa, including two novel species. Article Snippet: Amplification consisted of an initial denaturation at 95 C for 15 min, followed by 5 cycles of denaturation at 95 C for 30s, annealing at 50 C for 30s, and extension at 72 C for 1 min 30s, then an additional 35 cycles of denaturation at 95 C for 30s, annealing at 54 C for 30s, and extension at 72 C for 1 min 30s, with a final extension at 72 C for 10 min. PCR products were cleaned using EXOSAP-IT PCR Product Cleanup Reagent (Thermo Fisher Scientific, Waltham, Massachusetts) to remove residual chemicals. .. PCR products were sequenced in both directions according to the BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) protocol using the forward and reverse COI primers at an annealing temperature of 52 C. Sequencing PCR products were precipitated using the sodium acetate/ethanol method and submitted to the Article Title: First report of
<i>Melanterius inconspicuus</i>
(Coleoptera: Curculionidae: Cleogonini) from KwaZulu-Natal, South Africa, with observations of its development in black wattle Article Snippet: PCR products were sequenced according to the BigDye® Terminator v3.1 cycle sequencing kit (ThermoFisher Scientific) protocol in both the forward and reverse directions at an annealing temperature of 55 °C. .. Sequencing PCR products were precipitated using the sodium-acetate/ethanol method and submitted to the Sequencing:Article Title: Fusarium species associated with Euwallacea xanthopus in South Africa, including two novel species. Article Snippet: Amplification consisted of an initial denaturation at 95 C for 15 min, followed by 5 cycles of denaturation at 95 C for 30s, annealing at 50 C for 30s, and extension at 72 C for 1 min 30s, then an additional 35 cycles of denaturation at 95 C for 30s, annealing at 54 C for 30s, and extension at 72 C for 1 min 30s, with a final extension at 72 C for 10 min. PCR products were cleaned using EXOSAP-IT PCR Product Cleanup Reagent (Thermo Fisher Scientific, Waltham, Massachusetts) to remove residual chemicals. .. PCR products were sequenced in both directions according to the BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) protocol using the forward and reverse COI primers at an annealing temperature of 52 C. Sequencing PCR products were precipitated using the sodium acetate/ethanol method and submitted to the Article Title: First report of
<i>Melanterius inconspicuus</i>
(Coleoptera: Curculionidae: Cleogonini) from KwaZulu-Natal, South Africa, with observations of its development in black wattle Article Snippet: PCR products were sequenced according to the BigDye® Terminator v3.1 cycle sequencing kit (ThermoFisher Scientific) protocol in both the forward and reverse directions at an annealing temperature of 55 °C. .. Sequencing PCR products were precipitated using the sodium-acetate/ethanol method and submitted to the Article Title: Trehalose promotes biological fitness of fungi Article Snippet: For maize, a staple food in South Africa, there is a lack of comprehensive knowledge on the mycotoxin-producing fungal diversity.. In this study, a fungal community profile was established using culture-dependent methods for 56 maize seed samples that were also analysed for 13 mycotoxins.. The fungal isolates were identified by morphology and DNA sequencing. Article Title: Identification of Phytophthora cinnamomi CRN effectors and their roles in manipulating cell death during Persea americana infection Article Snippet: .. The supernatant was removed, and the DNA pellets were dried in a heating block set at 66 oC for 10 min. All samples were submitted to the Article Title: Global transcriptomic analysis in avocado nursery trees reveals differential gene expression during asymptomatic infection by avocado sunblotch viroid (ASBVd) Article Snippet: .. Products of sequencing reactions were purified using a sodium acetate precipitation before they were submitted to the Article Title: Identification of Phytophthora cinnamomi CRN effectors and their roles in manipulating cell death during Persea americana infection. Article Snippet: .. The supernatant was removed, and the DNA pellets were dried in a heating block set at 66 oC for 10 min. All samples were submitted to the Article Title: Global transcriptomic analysis in avocado nursery trees reveals differential gene expression during asymptomatic infection by avocado sunblotch viroid (ASBVd). Article Snippet: .. Products of sequencing reactions were purified using a sodium acetate precipitation before they were submitted to the Article Title: Diversity and distribution of turnip yellows virus infecting Brassica napus L. (canola) in the Western Cape, South Africa Article Snippet: Positive amplicons were purified using ExoSAP-ITTM (Thermo Fischer Scientific Baltics, Vilnius, Lithuania). .. Sequencing reactions were performed, in both forward and reverse directions, using a BigDyeTM Terminator v3.1 Cycle Sequencing Kit (Thermo Fischer Scientific Baltics, Vilnius, Lithuania), purified using ethanol/EDTA precipitation and submitted to the University of Pretoria, Blocking Assay:Article Title: Identification of Phytophthora cinnamomi CRN effectors and their roles in manipulating cell death during Persea americana infection Article Snippet: .. The supernatant was removed, and the DNA pellets were dried in a heating block set at 66 oC for 10 min. All samples were submitted to the Article Title: Identification of Phytophthora cinnamomi CRN effectors and their roles in manipulating cell death during Persea americana infection. Article Snippet: .. The supernatant was removed, and the DNA pellets were dried in a heating block set at 66 oC for 10 min. All samples were submitted to the Purification:Article Title: Global transcriptomic analysis in avocado nursery trees reveals differential gene expression during asymptomatic infection by avocado sunblotch viroid (ASBVd) Article Snippet: .. Products of sequencing reactions were purified using a sodium acetate precipitation before they were submitted to the Article Title: Global transcriptomic analysis in avocado nursery trees reveals differential gene expression during asymptomatic infection by avocado sunblotch viroid (ASBVd). Article Snippet: .. Products of sequencing reactions were purified using a sodium acetate precipitation before they were submitted to the Article Title: Diversity and distribution of turnip yellows virus infecting Brassica napus L. (canola) in the Western Cape, South Africa Article Snippet: Positive amplicons were purified using ExoSAP-ITTM (Thermo Fischer Scientific Baltics, Vilnius, Lithuania). .. Sequencing reactions were performed, in both forward and reverse directions, using a BigDyeTM Terminator v3.1 Cycle Sequencing Kit (Thermo Fischer Scientific Baltics, Vilnius, Lithuania), purified using ethanol/EDTA precipitation and submitted to the University of Pretoria, Electrophoresis:Article Title: Diversity and distribution of turnip yellows virus infecting Brassica napus L. (canola) in the Western Cape, South Africa Article Snippet: Positive amplicons were purified using ExoSAP-ITTM (Thermo Fischer Scientific Baltics, Vilnius, Lithuania). .. Sequencing reactions were performed, in both forward and reverse directions, using a BigDyeTM Terminator v3.1 Cycle Sequencing Kit (Thermo Fischer Scientific Baltics, Vilnius, Lithuania), purified using ethanol/EDTA precipitation and submitted to the University of Pretoria, |